Open AccessThe effect of sambung nyawa (Gynura procumbens) leaves extract to methyl mercury concentration in blood and characteristics of erythrocyte of rat after exposed by methyl mercury chloride
Author(s) -
Wiwik Widyawati,
Wiryanto Wiryanto,
Shanti Listyawati
Publication year - 2007
Publication title -
biofarmasi
Language(s) - English
Resource type - Journals
ISSN - 1693-2242
DOI - 10.13057/biofar/f050104
Subject(s) - mercury (programming language) , chloride , chemistry , hemoglobin , environmental chemistry , botany , traditional medicine , biochemistry , biology , medicine , organic chemistry , computer science , programming language
Widyawati W, Wiryanto, Listyawati S. 2007. The effect of sambung nyawa (Gynura procumbens) leaves extract to methyl mercury concentration in blood and characteristics of erythrocyte of rat after exposed by methyl mercury chloride. Biofarmasi 5: 26-37. Methyl mercury is the most toxic of the kind of mercury wich used society in various levels since a long ago. Naturally, methyl mercury is produced from methylation process of anorganic mercury by methanogenic bacteria on aquatic sediment. The over existency of methyl mercury in distric area will be toxic if entry the organisms body. The aims of the research were to study the effect of sambung nyawa (Gynura procumbens (Lour) Merr.) leaves extract to methyl mercury concentration in blood and the characteristics of erythrocyte of rat (Rattus norvegicus L.) after exposed by methyl mercury chloride, and to study the most effective dose of sambung nyawa (Gynura procumbens (Lour) Merr.) leaves extract to reduce the concentration of methyl mercury in blood and to repair the characteristics of erythrocyte after exposed by methyl mercury chloride. The research used a Completely Random Design with six groups, each group consisted of 4 repetitions. The treatments of each group were aquadest 2.5 ml (placebo), methyl mercury chloride 2.99 mg/kg BW (negative control), L-Cystein 5.4 mg/kg BW (positive control), and the extract of sambung nyawa leaves 1.945, 3.889, and 5.834 g/kg BW. The observation included the concentration of methyl mercury in blood, hemoglobin concentration, erythrocyte number, and Packed Cell Volume (PCV), which analyzed by Analysis of Variance (Anova) and continued with Duncan Multiple Range Test (DMRT) at significance level of 5%. The morphology of erythrocyte observation was analyzed qualitatively. The result of research showed that the extract of sambung nyawa leaves at doses of 1.945, 3.889, and 5.834 g/kg BW affected to reduce the concentration of methyl mercury in blood significantly and to repair the characteristics of erythrocyte. The extract of sambung nyawa leaves that had the highest effectiveness to reduce the methyl mercury concentration in blood and to repair the characteristics of erythrocyte was on a dose of 5.834 mg/kg BW.