Diuretic effect of milk coffee on white rat (Rattus norvegicus) with variation of milk types

Bistani DA, Listyawati S, Setyawan AD. 2007. Diuretic effect of milk coffee on white rat (Rattus norvegicus) with variation of milk types. Biofarmasi 5: 8-15. Caffeine content in coffee is a mild diuretic which increase glomerule filtration and reduce natrium reabsorbtion in renal tubule. Some people mix milk into the coffee because they do not like the bitter taste from caffeine. Milk contains glucose which can cause osmotic diuretic and increase urine excretion. The aim of this research was to find out the diuretic effects of orally intakes of coffee-milk on white male rats (Rattus norvegicus) with a variation of milk kind. This research was done in Biology Sub Laboratory, Central Laboratory of MIPA UNS, Surakarta, Central Java. A Completely Randomized Design with five groups and four replications to each group was used in this study. The treatments applied for those groups were: (i) aquadest (group I), (ii) coffee solution (group II), (iii) coffee + sweetened condensed milk solution (group III), (iv) coffee + soymilk solution (group IV), and (v) coffee + skim milk solution (group V). The parameters used for the physical characteristics of urine were volume, colour, clearness, pH value and density. The parameters used for the chemical characteristics were the glucose analysis by Benedict test for qualitative and spectrophotometry for quantitative, and the analysis of NaCl content by Fantus method. The data were analyzed by using the analysis of variance (Anova) and continued with Duncan Multiple Range Test (DMRT) at a significance level of 5%. The results showed that a variation of milk kind was not effect on volume, colour, clearness, pH value, density, and glucose content after 4 hours of treatments, but effect on NaCl content after 4 hours of treatments.