Open AccessReduction of exoprotease production in Aeromonas hydrophila by tomato fruits extract (Lycopersicon esculentum Mill.)
Author(s) -
Nur Aini,
Sutarno Sutarno,
Ari Susilowati
Publication year - 2006
Publication title -
biofarmasi
Language(s) - English
Resource type - Journals
ISSN - 1693-2242
DOI - 10.13057/biofar/f040203
Subject(s) - aeromonas hydrophila , quorum sensing , microbiology and biotechnology , bacteria , biology , lycopersicon , virulence , chemistry , biochemistry , botany , gene , genetics
Aeromonas hydrophila is pathogenic bacteria in fish. One of its virulence factors is exoprotease. The production of exoprotease is controlled by a quorum sensing system. Quorum sensing is an intercellular communication of bacteria that using a signal molecule of C4-HSL. The exoprotease production of A. hydrophila can be blocked by using quorum sensing inhibitors. The inhibition of quorum sensing of A. hydrophila can be conducted by C4-HSL analogs molecules. In this research, the molecules that predicted as quorum sensing inhibitors and act as C4-HSL analog molecules were furanone from tomato fruits. The inhibition of quorum sensing of A. hydrophila could be shown by a reduction of exoprotease production. The aim of this research was to determine the reduction of exoprotease production of A. hydrophila by extracts of n-hexane, ethyl acetate and methanol of tomato fruits with the concentrations of 0%, 2%, 4%, 6% and 8%, respectively. The qualitative exoprotease assay result showed that n-hexane extract of tomato had no effect on growth and exoprotease production of A. hydrophila. As much as 4% of ethyl acetate extract of tomato fruits could inhibit exoprotease production, but affect A. hydrophilla growth. Meanwhile, 4% methanol extract of tomato fruits could inhibit exoprotease production, without affect A. hydrophilla growth. The quantitative exoprotease assay result showed that 4% of methanol extract could inhibit exoprotease production by 71.68% without affect the growth of A. hydrophila.