Selection and identification of cellulolitic and lignocellulolitic fungi from organic waste of cajuput oil (Melaleuca leucadendron L.) from KPH Gundih, Regency of Grobogan

The aims of this research were isolating and selecting fungi that degrading cellulose and lignocellulose of organic waste from cajuput oil distillation. The research outline is organic waste from cajuput oil distillation contain cellulose and lignocellulose that cause naturally composting process take long time. The difficulty of organic waste decomposition depends on the matters in the tissue. To speed up decomposition process it need microorganism. Fungi have more ability to degrade cellulose and lignocellulose than bacteria. Because of that it was necessary to isolate and select fungi that able to degrade cellulose and lignocellulose of organic waste from cajuput oil distillation. This research was done by isolating and identifying fungi from organic waste from cajuput oil distillation and soil where organic waste from cajuput oil distillation was throw away. The selection cellulolytic fungi by inoculate fungi on CMC agar medium and to select lignocellulolytic fungi by inoculate soil dilution 10-4 to agar medium, which was added with organic waste from cajuput oil distillation. The result showed that total of 6 fungi were isolated from organic waste from cajuput oil distillation and soil where organic waste from cajuput oil distillation was throw away. Aspergillus parasiticus, A. nidulans, and Trichoderma harzianum have ability to degrade cellulose. Fungi that have ability to degrade lignocellulose of organic waste from cajuput oil distillation is A. parasiticus. A. parasiticus more potential degrade cellulose and lignocellulose organic waste from cajuput oil distillation than other fungi that found.