Larvicidal activity evaluation of the chemical compounds isolated from n-hexane extract of Derris elliptica root against the Temephos-susceptible strain of Aedes aegypti larvae

. Sayono S, Anwar R, Sumanto D. 2021. Larvicidal activity evaluation of the chemical compounds isolated from n-hexane extract of Derris elliptica root against the Temephos-susceptible strain of Aedes aegypti larvae. Biodiversitas 23: 757-764. Aedes aegypti is the main vector of Dengue, Chikungunya, and Zika diseases. Temephos resistance of this species has hampered vector control efforts worldwide. Studies proved that Derris elliptica extracts effectively controlled Aedes larvae, so the active compound needed to be isolated. This study evaluated the larvicidal activity of the chemical compounds isolated from n-hexane fractions of Tuba roots against the temephos-susceptible Ae. aegypti larvae. Six isolates were obtained from three of the seven n-hexane fractions. Three levels of concentration of each isolate were formulated for a preliminary bioassay test, and resulted in the two most active compounds, isolates 3 and 6. The final bioassay test results indicated that isolate 3 was more active than isolate 6 with LC50 and LC90 after 24 hours of exposure were 1.607 and 7.399 ppm, and after 48 hours of exposure were 0.926 and 3.206 ppm respectively. Isolate 6 also had high larvicidal activity after 48 hours of exposure, with LC50 and LC90 were 1.056 and 4.647 ppm. Further studies need to be carried out to determine the chemical structures and toxicity mechanisms of the bioactive compounds.