Open AccessDinoxin B Withanolide from Datura inoxia Mill as an Effective Phytocompound Against Urinary Tract Infection causing Staphylococcus aureus
Author(s) -
Ruby George,
Priti Mathur,
Chandni Tandon
Publication year - 2021
Publication title -
biomedical and pharmacology journal/biomedical and pharmacology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.191
H-Index - 18
eISSN - 2456-2610
pISSN - 0974-6242
DOI - 10.13005/bpj/2272
Subject(s) - withanolide , staphylococcus aureus , electrospray ionization , microbiology and biotechnology , antibacterial activity , penicillin binding proteins , minimum inhibitory concentration , chemistry , datura stramonium , penicillin , biology , bacteria , mass spectrometry , antimicrobial , chromatography , medicine , antibiotics , botany , alternative medicine , pathology , withania somnifera , genetics
Background: Dinoxin B Withanolide was isolated from Datura inoxia and identified with its cytotoxic activity. But its antibacterial properties are not yet evaluated. We have previously reported the broad-spectrum antibacterial property of Dinoxin B Withanolide extracted from D.inoxia on standard strains. Objective: This research has focused to evaluate the efficacy of Dinoxin B Withanolide against infectious Staphylococcus aureus, including resistant strains. Methods: Electrospray Ionization-Mass Spectrometry is used to depict the presence of Dinoxin B withanolide from the chromatographic ethanolic leaf fraction. Antibacterial activity of different concentrations of Dinoxin B(12500-1 μg/ml) was assessed using the agar diffusion, macro broth dilution, and time-kill assay methods. Docking studies and Drug likeness properties were analyzed. Result: Electrospray Ionization-Mass Spectrometry depicted the presence of Dinoxin B. All the isolates were susceptible to Dinoxin B within the range of 15±0.5mm to 24±0.5mm, and the bacteria were susceptible at a concentration rate of ≤12.5mg/ml. Time-kill assay showed that 25mg/ml of Dinoxin B displayed the highest inhibitory activity after four hours. The MBC values were compatible with the cidal concentration as seen in the time-kill study's growth curve. Computer-aided techniques resulted in a good Docking score towards Quorum-signaling Sar A protein (-7.82)and Penicillin Binding Protein(-6.9). Conclusion: Dinoxin B with its bactericidal properties and significant affinity towards Quorum-signaling Sar A protein and Penicillin Binding Protein can be considered as an effective bioactive compound against Methicillin Resistance Staphylococcus aureus.