Open AccessCorrelation between Sperm Dna Fragmentation and Conventional Semen Parameters among Different Age Groups
Author(s) -
Shruti Chopra,
Ajit Varma,
Seema Jain,
Sangeeta Jain,
Devendra Choudhary
Publication year - 2021
Publication title -
biomedical and pharmacology journal/biomedical and pharmacology journal
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.191
H-Index - 18
eISSN - 2456-2610
pISSN - 0974-6242
DOI - 10.13005/bpj/2235
Subject(s) - semen , dna fragmentation , sperm , andrology , semen analysis , sperm motility , staining , human fertilization , infertility , biology , medicine , apoptosis , anatomy , genetics , pregnancy , programmed cell death
Objective: To study the relationship between conventional semen parameters and sperm chromatin condensation (DNA fragmentation index) using aniline blue-eosin staining method among patients of different age groups visiting the In-vitro fertilization (IVF) clinic.Design: Retrospective study Setting: Tertiary care infertility centre Method: A total of 240 patient semen samples were studied between the period of May 2015 to May 2016 for conventional semen parameters (WHO criteria) and DNA fragmentation index (DFI) using aniline blue- eosin staining method. Patients were separated into three groups: 0.05). Conclusion: Our study demonstrated that age is not a predictor of DFI. Whereas, sperm concentration, sperm motility and normal sperm morphology showed a significant association with DFI in all the age groups i.e., better the conventional semen parameters, lower the DFI.