Open AccessAssessment of ACE A-240T Polymorphism with Chronic Kidney Disease in North Indian Population
Author(s) -
Narendra Mohan Verma,
Nayna Gupta,
Keshav Shukla,
Sanjeev Kumar Maurya
Publication year - 2018
Publication title -
biosciences biotechnology research asia/biosciences biotechnology research asia
Language(s) - English
Resource type - Journals
eISSN - 2456-2602
pISSN - 0973-1245
DOI - 10.13005/bbra/2708
Subject(s) - genotyping , genotype , kidney disease , allele , angiotensin converting enzyme , medicine , population , restriction fragment length polymorphism , polymorphism (computer science) , allele frequency , gastroenterology , biology , genetics , gene , blood pressure , environmental health
Chronic kidney disease (CKD) is a major public health problem with high risk of morbidity and mortality. Angiotensin converting enzyme (ACE) gene plays a significant role in the pathogenesis of chronic kidney disease (CKD) in different ethnic groups. This study aimed to investigate an association between ACE (A-240T) gene polymorphism and CKD in North Indian population. This case-control study was conducted in 385 subjects- 165 patients with CKD and 220 healthy controls. Genotyping of ACE A-240T polymorphism was performed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) method. The AA genotype and the A allele distributions were higher in both groups than the TT genotype and the T-allele. But, the genotypic and allelic distributions were not statistically significant difference between CKD patients and healthy controls. Also, no significant difference was found between the two groups in dominant, recessive and codominant genetic models. Our study suggested that the ACE A-240T variant not seems to be a risk factor for CKD in North Indian population. Further studies with a larger sample size are needed to confirm these results.