Open AccessIsolation, Identification and Characterization of Streptomyces sp. SN-2
Author(s) -
Bantanahal Talari Vishwanatha,
Girish Babu K,
Padmashri Swathi B Malagi,
Chethan Jambanna Dandin,
Sreenivasa Nayaka
Publication year - 2017
Publication title -
biosciences biotechnology research asia/biosciences biotechnology research asia
Language(s) - English
Resource type - Journals
eISSN - 2456-2602
pISSN - 0973-1245
DOI - 10.13005/bbra/2585
Subject(s) - 16s ribosomal rna , isolation (microbiology) , streptomyces , strain (injury) , biology , ribosomal rna , identification (biology) , gene sequence , microbiology and biotechnology , gene , botany , bacteria , genetics , anatomy
The present investigation is carried out for the isolation and molecular characterization of actinomycetes from the soil samples from Karnatak University campus, Dharwad, Karnataka, India. Totally four actinomycetes isolated, out of four isolated only one actinomycetes strain (SN-2) showed positive results for antimicrobial activity. SN-2 isolated actinomycetes was further analysed for morphological, physiological, biochemical and 16S ribosomal RNA gene sequencing. The SN-2 strain gene sequence was predicted with secondary structure and restriction sites were identified with the help of Genebee and NEBcutter tools. This investigation clearly indicates that the isolated strain SN-2 as Streptomyces sp.