Open AccessEffect of Surface Sterilization time and Plant Bioregulators for Callus Formation in Hybrid Lilium Cv. Tresor
Author(s) -
Anchal Arpita Gochhayat,
Sashikala Beura,
Enketeswara Subudhi
Publication year - 2017
Publication title -
biosciences biotechnology research asia/biosciences biotechnology research asia
Language(s) - English
Resource type - Journals
eISSN - 2456-2602
pISSN - 0973-1245
DOI - 10.13005/bbra/2498
Subject(s) - callus , lilium , explant culture , sterilization (economics) , bulb , horticulture , in vitro , biology , botany , chemistry , biochemistry , economics , monetary economics , foreign exchange market , foreign exchange
An efficient protocol was standardized for calli mass formation from bulb scale explant of hybrid Lilium Cv. Tresor under in vitro conditions at Biotechnology-cum-Tissue Culture Centre, OUAT, Bhubaneswar. The bulb scale explants were treated with 0.1 % HgCl2 (3 min, 4min, 5min, 6 min, 7min, 8min and 9 min) and control (without treatment) were cultured on MS media, among the treatments, 5 minutes timing resulted in minimum contamination [fungal % (6.67), bacterial % (6.67)] and maximum survival % (83.33%). The best surface sterilization time was further taken into consideration for treatment of explants, sterilization and cultured in the MS Basal media supplemented with BAP (0.5, 1.0 mg/l) in combination with 2,4-D (0.5,1.0,1.5,2.0, and 2.5 mg/l) and 2,4-D (0.5,1.0,1.5,2.0, and 2.5 mg/l) alone along with control. Basal media supplemented with BAP (1.0 mg/l) and 2,4-D (1.50 mg/l) produced maximum callus % (90.00%) and spread, profuse green callus was also recorded in similar combination which opened prospects for developing an indirect means of in vitro regeneration of hybrid Lilium Cv. Tresor there by strengthening the way biotechnology which could be used for improvement and satiate the national and international demands of this cut flowers.