Open AccessSterile Tissue Preparation and Callus Induction of Curcuma longa Linn.
Author(s) -
Warunya Kaewthip,
Srisulak Dheeranupattana,
Pornchai Junta,
Lalida Shank
Publication year - 2021
Publication title -
chiang mai university journal of natural sciences.
Language(s) - English
Resource type - Journals
ISSN - 2465-4337
DOI - 10.12982/cmujns.2021.062
Subject(s) - callus , curcuma , rhizome , explant culture , agar , murashige and skoog medium , zingiberaceae , chemistry , curcuminoid , botany , tissue culture , horticulture , traditional medicine , biology , in vitro , biochemistry , medicine , genetics , bacteria
Curcuma longa Linn. (family Zingiberaceae), commonly known as ‘turmeric’, is native to Southeast Asia. Turmeric has been used for color, flavor as a spice in cuisine and employed for treatment of various diseases. The major component in yellow-pigmented fraction of turmeric is curcuminoids. Curcuminoid production in callus of C. longa Linn. is our focus of study. Sterile techniques to obtain germ-free of C. longa Linn. explants were investigated and the results showed that immersing rhizome buds in 70% ethanol for 5 min, followed by 0.10% HgCl2 for 10 min offered approximately 66% survival rate. Multiple shoots were generated from the aseptic rhizome explants cultured on Murashige and Skoog (MS) agar medium fortified with 3.00 µM of 6-Benzylaminopurine (BA) and 0.50 µM of 1-Naphthaleneacetic acid (NAA) at 25 ± 2°C under a photoperiod of 16 h light and 8 h dark. The sterile leaf sheath and root were subsequently used for callus induction which produced various responses when cultured on MS agar medium fortified with different concentrations of 2,4-dichlorophenoxy acetic acid (2, 4-D), Thidiazuron (TDZ) and BA. The highest induction yields of friable callus were obtained from leaf sheath segments cultured on MS agar medium fortified with 0.50 mg/l 2, 4-D which are the conditions proposed for successful production of callus culture of C. longa Linn. Keywords: Callus induction, Curcuma longa Linn., Turmeric, Plant tissue culture