Open AccessEvaluation of leishmanicidal effect of Euphorbia erythadenia extract by in vitro leshmanicidal assay using promastigotes of Leishmania major
Author(s) -
Reza Kazemi Oskuee,
Mahmoud Reza Jaafari,
Sara Amani,
Mohammad Ramezani
Publication year - 2014
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.12980/apjtb.4.2014c1018
Subject(s) - euphorbia , ec50 , traditional medicine , extraction (chemistry) , chemistry , chromatography , leishmania , positive control , solvent , in vitro , incubation , negative control , medicine , biochemistry , parasite hosting , world wide web , computer science
ABSTRACTObjectiveTo evaluate leishmanicidal effects of Euphorbia erythadenia plant extract.MethodsExtraction was done using methanolic Soxhlet of dried and ground aerial parts of the plant. Then, five different extract concentrations, in addition of positive, negative and solvent controls were prepared and added to a 24-well plate containing 40 000 parasites/well. The extract concentrations were 1, 0.5, 0.25, 0.125 and 0.062 5 mg/mL. Amphotricin B (0.5 mg/mL) was used as positive control while negative control contained only culture medium. After 3 d incubation at 25 °C the amount of parasites in each well was determined on each day of experiment microscopially using Neubar chamber.ResultsSoxhlet extract as well as amphotricin B killed all parasites at concentration of 1 mg/mL. The leshmanicidal activity of lower doses of extract was dose-dependent. The EC50 for Soxhlet extracts in dimethylsulfoxide was 0.30 mg/mL. The EC50 for Soxhlet extracts in methanol was 0.23 mg/mL. No obvious effects from the control solvent on the Leishmania major promastigotes were observed.ConclusionsThe Soxhlet extract of Euphorbia erythadenia showed suitable leishmanicidal activity, especially in higher concentration fractions