Open AccessIdentification of sitosteryl glucoside palmitate in a chloroform-derived fraction of Phyllanthus niruri with antiplasmodial and peripheral antinociceptive properties
Author(s) -
Ifeoma Ezenyi,
Kulkarni Roshan,
Joshi Swati,
Salawu Oluwakanyinsola Adeola,
Martins Emeje
Publication year - 2014
Publication title -
asian pacific journal of tropical biomedicine/asian pacific journal of tropical biomedicine
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.507
H-Index - 61
eISSN - 2588-9222
pISSN - 2221-1691
DOI - 10.12980/apjtb.4.2014apjtb-2013-0025
Subject(s) - phyllanthus , chloroform , chemistry , glucoside , cytotoxicity , ic50 , chromatography , methanol , traditional medicine , anti inflammatory , acetic acid , column chromatography , ethyl acetate , in vitro , pharmacology , biochemistry , medicine , organic chemistry , alternative medicine , pathology
ObjectiveTo evaluate the antiplasmodial properties of fractions of chloroform portion of Phyllanthus niruri (P. niruri) methanol extract and identify a suitable chemical marker present therein.MethodsChloroform portion of P. niruri methanol extract was separated from silica gel using gradient systems of hexane, ethylacetate and methanol. The fractions were screened for antiplasmodial activity against Plasmodium falciparum HB3 and FcM29. Fractions with IC50<10 μg/mL against parasites were further screened for peripheral analgesic activity, while cytotoxicity was evaluated using THP-1 cells.ResultsFractions 12-14 were very active (IC50<10 μg/mL) against Plasmodium falciparum and showed no significant cytotoxicity. Fractions 12 and 13 exhibited significant (P<0.01) reduction in acetic acid-induced writhing in mice, decreasing the number of writhes by 66.67% and 65.22% respectively and comparable with 100 mg/kg aspirin (65.22%). From fraction 12, a compound was isolated and identified as sitosteryl-6-β-D-glucoside-6’-palmitate by 1H, 13C nuclear magnetic resonance and mass spectroscopies.ConclusionsOur findings illustrate antiplasmodial column fractions of P. niruri with analgesic activity and identify sitosteryl glucoside palmitate as a chemical marker of activity