Open AccessDetermination of coumarins from aerial part of two Artemisia species
Author(s) -
Marcin Berger,
Jolanta Szymańska
Publication year - 2015
Publication title -
current issues in pharmacy and medical sciences
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.134
H-Index - 9
eISSN - 2300-6676
pISSN - 2084-980X
DOI - 10.12923/j.2084-980x/26.1/a.14
Subject(s) - artemisia , scopoletin , herb , chromatography , extraction (chemistry) , high performance liquid chromatography , botany , traditional medicine , coumarin , medicinal herbs , chemistry , medicine , biology , alternative medicine , pathology
The methanolic and methanolic-water extracts were received from a blooming aerial part (herb) of two species: Artemisia gmelinii Webb ex Stechmann and Artemisia umbelliformis Lam. Two methods were used for extracting the plant material: Accelerated Solvent Extraction (ASE) and Soxhlet Extraction. The analysis of samples was performed by the HPLC-DAD method. All obtained extracts were subjected to the process of the solid phase extraction. Samples for HPLC-DAD analysis were purified using C-18 (Octadecyl, 500 mg) Baker Bond (J.T. Baker, USA) SPE-micro-columns. The quantitative indications of individual constituents (coumarins) were converted into the content of scopoletin.