Open AccessEffect of NOx on the somatic chromosomes of goldsmiths.
Author(s) -
J. S. Yadav,
Neena Seth
Publication year - 1998
Publication title -
environmental health perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.257
H-Index - 282
eISSN - 1552-9924
pISSN - 0091-6765
DOI - 10.1289/ehp.98106643
Subject(s) - nox , sister chromatid exchange , sister chromatids , mitotic index , chromosome , somatic cell , microbiology and biotechnology , genetics , biology , chemistry , mitosis , in vitro , organic chemistry , gene , combustion
The genotoxic effect of NOx was investigated on somatic human chromosomes obtained from lymphocytes of 45 goldsmiths exposed to 1770.5 mg/m3 NOx in ambient air at normal temperature and pressure and compared to an equal number of matched controls breathing air containing 50 microgram/m3 NOx. Short-term lymphocyte cultures were set up from blood collected from both exposed and control individuals by venipuncture in heparinized sterile syringes. Mitotic index (MI), chromosome aberrations (CAs), sister chromatid exchanges (SCEs), and satellite associations (SAs) were analyzed. All the parameters showed a significant increase (p<0.01 and p<0.05) in the exposed individuals as compared to the controls: MI (9.57 vs. 5.01), CAs (3. 48 vs. 0.711), SCEs (10.56 vs. 7.02), and SAs (25.97 vs. 12.84), respectively. Occurrence of DG-type SAs (one D-group chromosome and one G-group chromosome) was highest and 3 D-type (three D-group chromosomes) lowest. NOx was thus found to be genotoxic for humans.