Open AccessChemical models important in understanding the ways in which chromate can damage DNA.
Author(s) -
Paul O’Brien,
Andreas Kortenkamp
Publication year - 1994
Publication title -
environmental health perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.257
H-Index - 282
eISSN - 1552-9924
pISSN - 0091-6765
DOI - 10.1289/ehp.94102s33
Subject(s) - chromate conversion coating , reactivity (psychology) , chemistry , dna damage , carcinogen , dna , toxicity , glutathione , toxicology , biochemistry , enzyme , biology , medicine , organic chemistry , pathology , chromium , alternative medicine
Chromate is an established human carcinogen. There have been many studies of the reactivity of chromate aimed at improving understanding of chromate toxicity. In the present paper a number of conclusions of these studies are reviewed and considered in the light of new results obtained in our laboratories. A number of hypotheses are considered; it is concluded, however, that it is impossible to reconcile the generation of strand breaks by chromate during its reduction by glutathione with any simple mechanism involving the generation of DNA lesions by free hydroxyl radicals. Kinetic, spin-trapping, and competition kinetic studies, based on a strand-breaking assay, are reported in support of this conclusion.