Open AccessAllogeneic transplantation of normal epidermal cells and squamous cell carcinomas in SENCAR mice.
Author(s) -
Claudio J. Conti,
Jo Ann W. Fries,
C. Marcelo Aldaz,
Andres J. KleinSzanto,
Thomas J. Slaga
Publication year - 1986
Publication title -
environmental health perspectives
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.257
H-Index - 282
eISSN - 1552-9924
pISSN - 0091-6765
DOI - 10.1289/ehp.8668125
Subject(s) - transplantation , epidermis (zoology) , pathology , cell , malignancy , basal cell , chemistry , suspension culture , cell culture , medicine , biology , anatomy , biochemistry , genetics
A method used in our laboratory for the transplantation of single cell suspensions of epidermis and skin carcinomas is described. Silicone chambers were placed into a subcutaneous granulation tissue formed by the implantation of a glass disk. The skin was closed on top of the chamber by using Michael autoclips. Cells were injected 24 to 48 hr later through the skin using a syringe and needle. The neoformation of epithelia with adnexa was observed when newborn epidermal cells were injected. When a fresh cell suspension of squamous cell carcinoma was used, a typical differentiated carcinoma was formed within 2 to 4 weeks. However, after 4 weeks, some of the grafts showed mild signs of rejection. The technique described is a useful system to transplant squamous cell carcinomas and can also be used as a rapid assay for malignancy.