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Expression of a cell‐cycle‐associated nuclear antigen (MIB 1) in cholesteatoma and auditory meatal skin
Author(s) -
Sudhoff Holger,
Hildmann H.,
FisselerEckhoff Annette,
Bujía Jesus,
Holly Anja,
SchulzFlake Christian
Publication year - 1995
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199511000-00017
Subject(s) - cholesteatoma , pathology , immunohistochemistry , stroma , biology , medicine , radiology
Middle ear cholesteatoma is often invasive with consequent bone destruction. Inflammatory stimulation of the underlying connective tissue, as well as an autocrine mechanism, may be responsible for the dys‐regulation and abnormal proliferative features of the keratinocytes in cholesteatoma. Comparative investigations were performed to assess the epithelial cell kinetics of cholesteatoma and normal auditory meatal skin. Monoclonal antibody MIB 1 immuno‐staining (which recognizes a nuclear antigen expressed by dividing cells) was applied using the alkaline phosphatase antialkaline phosphatase im‐munolabeling method. Specimens of normal auditory meatal skin (n = 7) revealed an average MIB 1 score (quotient of the MIB 1‐positive cells and the total number of cells) of 7.6 ± 2.2%. Cholesteatoma samples (n = 13) showed an average MIB 1 score of 17.4 ± 8.9% and a heterogeneity of proliferating epithelial areas. Epithelial cones growing toward the underlying stroma exhibited high mitotic activity. Statistically, the results of this study confirm a highly significant increase in the proliferation rate of cholesteatoma keratinocytes, which had an MIB 1 score that was 2.3 times higher than the score for keratinocytes of normal external auditory meatal skin.