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Topoisomerase I activity in squamous cell carcinoma of the head and neck
Author(s) -
Masin Jeffrey S.,
Berger Sosamma J.,
Setrakian Sebouh,
Stepnick David W.,
Berger Nathan A.
Publication year - 1995
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199511000-00010
Subject(s) - topoisomerase , head and neck squamous cell carcinoma , basal cell , head and neck , carcinoma , cell , cancer research , medicine , biology , pathology , head and neck cancer , enzyme , cancer , biochemistry , surgery
In recent preclinical and clinical trials, topoisomerase I inhibitors have shown great promise as antitumor agents. These agents are most effective against tumors with high topoisomerase I activity. Therefore, determining topoisomerase I activity in advance may predict response to topoisomerase I inhibitors. Squamous cell carcinoma of the head and neck and normal tissue samples were obtained from 12 patients. Cellular extracts were prepared, and topoisomerase I activity assays were performed. The results suggest that topoisomerase I activity in squamous cell carcinoma of the head and neck is increased approximately sixtyfold compared to normal tissue. Increased activity often correlates with clinical responsiveness; these results predict that topoisomerase I inhibitors should be effective and selective against squamous cell carcinoma of the head and neck.