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A tissue‐culture model for the study of canine vocal fold fibroblasts
Author(s) -
Broadley Caroline,
Gonzalez Debra A.,
Nair Rhada,
Koriwchak Michael J.,
Ossoff Robert H.,
Davidson Jeffrey M.
Publication year - 1995
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199501000-00008
Subject(s) - elastin , lamina propria , elastase , fibroblast , wound healing , pathology , tissue culture , vocal folds , chemistry , biology , anatomy , epithelium , medicine , larynx , enzyme , immunology , biochemistry , in vitro
A tissue‐culture model has been developed for the study of fibroblasts from the canine vocal fold. Laryngeal tissue (lamina propria) obtained from euthanized dogs is rinsed, cut into 1‐mm 3 pieces, and incubated in 5% carbon dioxide at 37° C. A confluent monolayer is established within several days. Detectable levels of elastin in the tissue culture supernatant are measured by an indirect enzyme‐linked immunosorbent assay. Various external agents have been shown to affect elastin production. The effects of KTP laser irradiation, hydrocortisone (1.3 μmol/L), transforming growth factor‐β (10 ng/mL), and human leukocyte elastase have been measured. Thus the canine vocal fold fibroblast tissue culture is established as a model for further investigations to improve wound healing and to understand the wound‐healing process following laryngeal microsurgery.