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Cloning and sequencing of genomic DNA extracted from archival human temporal bone sections
Author(s) -
Kerner Marc M.,
Wackym Phillip A.,
Popper Paul,
Tabor David E.,
Grody Wayne W.
Publication year - 1994
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199402000-00002
Subject(s) - dna sequencing , cloning (programming) , biology , dna , molecular cloning , computational biology , mitochondrial dna , genetics , genomic dna , gene , complementary dna , computer science , programming language
Cloning techniques allow the engineering and production of highly purified DNA. Further advances in molecular biology have provided the means to identify DNA sequences in a rapid fashion. Sequencing methods can identify mutations, deletions, polymorphisms, or confirm a known genetic sequence. The use of these techniques in clinical medicine has made it possible to accurately diagnose infectious diseases and determine the molecular etiology of many genetic disorders and malignancies. In this study, DNA extracted from archival, celloidin‐embedded temporal bone sections has been cloned and sequenced using these techniques. We amplified, cloned, and sequenced varicella‐zoster viral DNA extracted from archival temporal bone sections from patients who had herpes zoster oticus. The application of cloning and sequencing techniques to DNA extracted from archival temporal bones provides the methodology to study temporal bone pathology at the molecular level.