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Localization of glucocorticoid receptors and glucocorticoid receptor mRNAs in the rat cochlea
Author(s) -
Ten Cate WJ. F.,
Curtis L. M.,
Rarey K. E.,
Small G. M.
Publication year - 1993
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199308000-00007
Subject(s) - spiral ganglion , spiral ligament , glucocorticoid receptor , organ of corti , cochlea , riboprobe , in situ hybridization , receptor , immunocytochemistry , biology , microbiology and biotechnology , medicine , endocrinology , messenger rna , anatomy , biochemistry , gene
The distribution of glucocorticoid (GR) receptor messenger RNAs (mRNAs) and GR receptors was studied by in situ hybridization histochemistry and immunocytochemistry, respectively. In situ hybridization histochemistry was performed with a biotin‐labeled riboprobe complementary to rat GR receptor mRNA. GR receptor mRNAs were demonstrated in spiral ligament cells, spiral limbus cells, and spiral ganglion cells. GR receptor mRNAs were demonstrated neither in cells of the stria vascularis nor in cells of the organ of Corti region. With the use of a monoclonal and a polyclonal antibody, GR receptors were observed in the spiral ligament cells, stria vascularis cells, spiral limbus cells, and spiral ganglion cells by immunocytochemistry. Binding of anti‐GR‐receptor antibodies to a lesser extent was observed in the organ of Corti region; however, cellular distribution of the GR receptors could not be resolved with the applied techniques. These results suggest that the GR receptor is expressed differently in the heterogeneous cochlear tissues.