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Successful in vitro growth of human respiratory epithelium on a tracheal prosthesis
Author(s) -
Chopra Dharam P.,
Kern Robert C.,
Mathieu Patricia A.,
Jacobs John R.
Publication year - 1992
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199205000-00011
Subject(s) - prosthesis , stenosis , anastomosis , tracheal stenosis , respiratory epithelium , medicine , epithelium , surgery , respiratory system , anatomy , pathology , radiology
Reconstruction of tracheal defects may be necessary following trauma or oncologic surgery. Defects up to 8 cm can often be repaired using end‐to‐end anastomosis. Use of a tracheal prosthesis for larger defects has been complicated by recurrent stenosis and infection. Recent animal studies, utilizing a Dacron® polyurethane prosthesis suggest that problems with anastomotic stenosis and infection can be controlled. Problems with a central stenosis within the prosthesis persist when used for defects greater than 6 cm. Establishment of a confluent lining of respiratory epithelium is believed to be necessary for successful prosthetic tracheal reconstruction. Using cell culture techniques, we report the first successful seeding and growth of human respiratory epithelium onto a Dacron polyurethane tracheal prosthesis.