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Hyaluronan synthesis in the adult guinea pig endolymphatic sac
Author(s) -
Parker David A.,
Schindler Robert A.,
Amoils C. Philip,
Lustig Lawrence R.,
Hradek Gary T.
Publication year - 1992
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-199202000-00009
Subject(s) - endolymphatic sac , endolymph , guinea pig , glycosaminoglycan , inner ear , connective tissue , in vitro , chemistry , anatomy , secretion , endolymphatic hydrops , microbiology and biotechnology , biology , pathology , biochemistry , endocrinology , medicine , meniere's disease , disease
The endolymphatic sac is believed to play a major role in membranous labyrinth homeostasis by controlling the volume of endolymph, removing debris, and participating in the immune response of the inner ear. The endolymphatic sac is postulated to absorb endolymph and to synthesize and secrete high‐molecular‐weight and osmotically active glycosaminoglycans (GAGs). The present study examines the ability of in vitro adult guinea pig endolymphatic sac cells to synthesize complex proteins and polysaccharides. The intent is to characterize the nature of these compounds by studying carbon‐14 ( 14 C) glucose incorporation in tissue cultured endolymphatic sac specimens using autoradiographic and specific enzymatic digestion techniques. Our results suggest that sac cells can synthesize GAGs and proteins in vitro in proportionately larger amounts than surrounding connective tissue and dura. The principal GAG synthesized by the endolymphatic sac appears to be hyaluronan.