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Further studies on growth characteristics of epstein‐barr virus (EBV) genome‐positive and ‐negative epithelial hybrid cell lines
Author(s) -
Takimoto Toru,
Tanaka Saichiroh,
Ishikawa Shigeru,
Masuda Koh,
Umeda Ryozo
Publication year - 1989
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-198907000-00015
Subject(s) - biology , virology , epstein–barr virus , virus , genome , genetics , gene
Differences in tumorigenicity between the Epstein‐Barr virus (EBV) genome‐negative and ‐positive epithelial A2L/AH hybrid cell lines have been described recently. Further differences in growth characteristics of the two epithelial hybrid cells have since been examined. Differences were found in doubling time and cloning efficiency in agarose of the two cell lines. In addition, the EBV genome‐negative epithelial hybrid cell line (cl‐654), unlike the EBV genome‐positive epithelial hybrid cell line (cl‐1), failed to grow in the unmanipulated nude mice, but was successfully transplanted in 60 Co‐irradiated nude mice. The histopathologic findings of tumors induced by the inoculation of cl‐654 cells in nude mice showed more differentiated carcinoma than the findings of tumors induced by cl‐1 cells. The data suggest that EBV is associated with an alteration of cell differentiation in epithelial cells as well as tumorigenicity in nude mice.