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Bioinhibition of human fibroblast cultures sensitized to Q‐switch II dye and treated with the Nd:YAG laser: A new technique of photodynamic therapy with lasers
Author(s) -
Castro Dan J.,
Ward Paul H.,
Saxton Romaine E.,
Fetterman Harold R.,
Castro Donna J.
Publication year - 1989
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-198904000-00012
Subject(s) - fibroblast , laser , connective tissue , dye laser , photodynamic therapy , materials science , scars , wound healing , phototoxicity , biomedical engineering , dermal fibroblast , biophysics , chemistry , medicine , surgery , optics , pathology , in vitro , biochemistry , organic chemistry , physics , biology
Kodak Q‐switch II dye has recently proven to be nn effective biostimulative agent on normal human fibroblast cultures. The potential for this dye as a new chemosensitizing agent for the treatment of connective tissue diseases and wound healing with the Nd:YAG laser was examined. Two normal fibroblast cell lines were first sensitized to a nontoxic dose of Q‐switch II dye, then subjected to treatment with an Nd:YAG laser at 1, 060 nm, with varying levels of energy and temperatures determined by a reproducible method of dosimetry. The results indicate that Q‐switch II dye at nontoxic doses of 0.1 μg/ml enhances the cytotoxic effects of the Nd:YAG laser at temperatures as low as 36 °C. Furthermore, at physiological temperature ranges as low as 24 °C to 34 °C, cell duplication was inhibited, but cell viability was not affected. Similar results were not observed when fibroblast cultures were treated with the laser alone. These observations suggest that Q‐switch II dye is an effective chemosensitizing agent for the Nd:YAG laser and could potentially be used to reduce collagen deposits in conditions such as keloids and hypertrophic scars.