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In Situ hybridization and immunohistochemical study of human papillomavirus infection in adult laryngeal papillomas
Author(s) -
Tsutsumi Kouichiro,
Nakajima Takashi,
Gotoh Masahiro,
Shimosato Yukio,
Tsunokawa Youko,
Terada Masaaki,
Ebihara Satoshi,
Ono Isamu
Publication year - 1989
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-198901000-00015
Subject(s) - in situ hybridization , papilloma , immunohistochemistry , antigen , biology , human papillomavirus , papillomaviridae , pathology , hpv infection , hybridization probe , dna , virology , messenger rna , medicine , gene , cancer , immunology , cervical cancer , genetics
Routinely processed paraffin sections from 20 patients with adult laryngeal papillomas were examined for the presence of human papillomavirus type 11 (HPV‐11) DNA and its specific mRNA by in situ hybridization methods using 35 S‐labeled RNA probes. Immunohistochemical techniques were also used to identify papillomavirus genus‐specific common antigen (pgsantigen). HPV‐11 DNA signals and/or papillomavirus genus‐specific common antigen were detected in all eight samples of multiple laryngeal papilloma. On the other hand, in 12 samples of single laryngeal papilloma, neither papillomavirus genus‐specific common antigen nor HPV‐11 DNA were detected. Four patients were positive for both HPV‐11 DNA and pgs‐antigen. In three of these four patients, HPV‐11 mRNA signals were also detected. These results provided direct evidence of the association of HPV and adult multiple laryngeal papilloma.