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Biostimulative effects of ND:YAG q‐switch dye on normal human fibroblast cultures: Study of a new chemosensitizing agent for the ND:YAG laser
Author(s) -
Castro Dan J.,
Ward Paul H.,
Saxton Romaine E.,
Castro Donna J.,
Fetterman Harold R.
Publication year - 1987
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-198712000-00014
Subject(s) - trypan blue , fibroblast , viability assay , connective tissue , cell culture , cell , wound healing , microbiology and biotechnology , biophysics , chemistry , andrology , materials science , biomedical engineering , in vitro , medicine , surgery , pathology , biochemistry , biology , genetics
Kodak Q‐switch II® is a new chemical with an absorption maxima at 1,051 nm, designed to be used as an Nd:YAG dye laser. The potential for this dye as a new chemosensitizing agent in the treatment of connective tissue diseases and wound healing with low energy Nd:YAG laser was examined. Two normal fibroblast cell lines were tested for sensitivity to various levels of this dye in vitro. These cells were exposed to Q‐switch II dye at concentrations of 0.01, 0.1, 1, 10, 50, and 100 μg/ml for 1 and 24 hours. Cell viability was assessed by the trypan blue exclusion test. Cell duplication and DNA synthesis were measured by the incorporation of [ 3 H]‐thymidine at 6 and 24 hours postexposure to Q‐switch II dye. At concentrations up to 10 μg/ml, both cell lines tested showed no changes in cell viability. However, at concentrations equal or higher than 50 μg/ml, more than 40% of the fibroblasts incorporated trypan blue after 24 hours of exposure to this dye, indicating significant cell destruction. The results indicate that Q‐switch II dye is nontoxic to normal human fibroblast cultures and showed significant biostimulative effects on cell duplication at concentrations equal to or lower than 10 μg/ml. Further studies will be required to determine the usefulness of Q‐switch EC dye as a new photochemosensitizing agent for potential biostimulation of wound healing and/or treatment of connective tissue diseases with the Nd:YAG laser (near infrared, 1,060 nm) at “nonthermal” levels of energies.