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Steep gradients of amino acids between cochlear endolymph and perilymph
Author(s) -
Thalmann R.,
Comegys T. H.,
Demott J. E.,
Thalmann I.
Publication year - 1981
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-198111000-00001
Subject(s) - perilymph , endolymph , cochlea , chemistry , high performance liquid chromatography , chromatography , amino acid , inner ear , biophysics , anatomy , biochemistry , biology
Levels of 19 free amino acids in cochlear endolymph and perilymph of scala vestibuli of the guinea pig were determined using reverse phase high performance liquid chromatography (HPLC) of the o‐phthaldialdehyde‐ethanethiol derivatives with fluorescence detection. Aspartate and glutamate were found to be significantly higher in endolymph than in perilymph, confirming results from another study using a different analytical method. The remaining 17 amino acids were significantly lower in the endolymph, in many cases by an order of magnitude. The data are compared with results on utricular endolymph and vestbular perilymph obtained by HPLC in another study from our laboratory. Possible implications and interpretations of the results are discussed.