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Quantitative determination of alkaline phosphatase and inorganic phosphate in fetal and neonatal otic capsules of rats
Author(s) -
Marovitz William F.,
Black Martin J.
Publication year - 1974
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-197404000-00013
Subject(s) - alkaline phosphatase , fetus , phosphatase , phosphate , capsule , inorganic phosphate , chemistry , electrophoresis , quantitative analysis (chemistry) , biochemistry , starch , embryonic stem cell , chromatography , microbiology and biotechnology , biology , enzyme , pregnancy , genetics , botany , gene
A quantitative assay technique for alkaline phosphatase in pooled fetal ears has been developed. The data obtained by this microchemical assay agrees well with previous histochemical techniques. Starch gel electrophoresis was unable to differentiate different alkaline phosphatases in the extract. These data should prove useful for examination of experimental pathology of the embryonic capsule.