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Introduction to the scanning electron microscope for the clinician‐researcher
Author(s) -
Lucente Frank E.,
Finke Edward H.,
Kaufman Arenberg I.
Publication year - 1973
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-197301000-00008
Subject(s) - scanning electron microscope , raster scan , raster graphics , electron beam induced deposition , optics , electron , materials science , environmental scanning electron microscope , physics , scanning transmission electron microscopy , computer science , computer graphics (images) , quantum mechanics
The scanning electron microscope is an exciting instrument which is easily adapted by the clinician‐researcher to the study of tissues of otolaryngologic interest. The SEM is based on the television raster principle of scanning a surface with a thin beam of electrons. As a result of the interaction of the electron beam with the surface, a number of physical phenomena are elicited including the emission of secondary electrons. The latter are collected and used to produce a synchronous visual representation of the specimen's surface. The photomicrograph obtained more closely resembles a three‐dimensional view than those obtained with light or phase microscopy. With recent refinements in the techniques of specimen preparation the spectrum of applications of the SEM continues to broaden.