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Fundamental aspects of incus transplantation
Author(s) -
Kuijpers W.,
Van Der Broek P.
Publication year - 1972
Publication title -
the laryngoscope
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.181
H-Index - 148
eISSN - 1531-4995
pISSN - 0023-852X
DOI - 10.1288/00005537-197212000-00005
Subject(s) - incus , ossicles , transplantation , homologous chromosome , medicine , resorption , middle ear , temporal bone , anatomy , surgery , biology , pathology , stapes , biochemistry , gene
In the present study, the results of experimental incus transplantations performed in a large number of rats are reported. The behavior of fresh autologous and homologous incudes and of incus grafts denatured by boiling or by preservation in alcohol, were studied up to survival times of more than two years. The incudes were transplanted orthotopically in the middle ear and heterotopically in the musculus tibialis anterior. Both fresh autologous and homologous incus grafts appeared to be well tolerated by the middle ear, although a substantial loss of osteocytes, most marked in the homografts, was observed after two years. No clear signs pointing to a transplantation reaction were observed in the homologous series. The denatured incudes appeared to retain their original shape in the middle ear after an observation period of more than two years, while in 40 percent of those incudes preserved in alcohol, new bone formation was observed. After heterotopical transplantation in muscle a clear transplantation reaction toward the fresh homologous incudes was found, while the autologous ossicles grossly retained their original shape and vitality. Extensive resorption was observed in the denatured incudes, although in the case of alcohol preserved ossicles, all specimens also showed areas of new bone formation. These experiments confirm the clinical evidence that the middle ear can be considered as one of the privileged sites of the body where transplantation reactions are absent or at least mitigated. Besides this, the results obtained with denatured incudes strongly suggest that new bone formation in the ossicles seems dependent not only upon the transplantation of osteogenic cells, but presumably also on the condition of the ground substance and or the presence of certain enzymes in the ossicle.