Open AccessFEATURES OF APOPTOSIS, FORMATION OF MEMBRANE-RELEASED MICROPARTICLES AND CYTOKINES PRODUCTION IN CASE OF LYMPHOCYTE DAMAGE IN SYNTROPY OF BRONCHIAL ASTHMA AND OBESITY IN YOUNG PATIENTS
Author(s) -
И. А. Соловьева,
И. А. Соловьева,
Ирина Демко,
Ирина Демко,
Е. А. Собко,
Е. А. Собко,
Н. А. Малиновская,
N. V. Malinovskaya,
А. Yu. Kraposhina,
А. Yu. Kraposhina,
Н. В. Гордеева,
Н. В. Гордеева
Publication year - 2019
Publication title -
bûlletenʹ fiziologii i patologii dyhaniâ
Language(s) - English
Resource type - Journals
ISSN - 1998-5029
DOI - 10.12737/article_5c88b4c63347f0.49183193
Subject(s) - adipokine , medicine , asthma , adiponectin , obesity , apoptosis , body mass index , immunology , lymphocyte , leptin , inflammation , endocrinology , insulin resistance , chemistry , biochemistry
The aim of the research was to study the characteristics of apoptosis, membrane-released microparticles (MRM) formation and cytokines production in case of lymphocyte damage in young patients with bronchial asthma, depending on body mass index. 224 people were examined. In all the patients there was done an assessment of the excessive weight and determination of obesity degree as well as clinical and functional examination; there was performed a determination of cytokines and adipokines; the lymphocytes were separated from the peripheral blood. The number of apoptotic cells was estimated in accordance with the standard protocol; the number of MRM was counted. It was found out that in case of bronchial asthma and obesity syntropy, even at a young age, more often there are patients with moderate and severe clinical course; besides, the severity of bronchial-obstructive syndrome in patients of this group is more significant with the tendency to decrease in FEV1 and FEV1/FVC. In the group of young patients with obesity combined with bronchial asthma in the peripheral blood there was imbalance of both adipokines and cytokines, characterized by evident increase in the levels of pro-inflammatory leptin, IL-4, IL-6, TNF-α, and the decrease in potential body weight regulator IL-15, and at the same time low levels of anti-inflammatory adiponectin. In patients with obesity, the percentage of cells in apoptosis was significantly lower compared to both those in control with obesity and patients with normal body weight. The number of MRM, as cell damage indicator, significantly increased in the group with obesity. It can be assumed that the imbalance of cytokines, adipokines and mechanisms of programmed cell death is a possible pathogenetic factor in asthma and obesity syntropy.