Open AccessDetermination of fenbendazole and its metabolites in milk by the method of liquid chromatography coupled with tandem mass-spectrometry
Author(s) -
П. П. Кочетков,
А И Варламова,
В. Е. Абрамов,
Н С Мисюра,
Ekaterina Abramova,
С. В. Абрамов,
Н И Кошеваров,
И. А. Архипов
Publication year - 2016
Publication title -
rossijskij parazitologičeskij žurnal
Language(s) - English
Resource type - Journals
eISSN - 2541-7843
pISSN - 1998-8435
DOI - 10.12737/23082
Subject(s) - fenbendazole , chromatography , chemistry , column chromatography , mass spectrometry , tandem mass spectrometry , liquid chromatography–mass spectrometry , metabolite , medicine , anthelmintic , biochemistry , veterinary medicine
Objective of research: Development of methods for the determination of fenbendazole and its metabolites in milk by liquid chromatography coupled with tandem mass spectrometry.
Materials and methods: Fenbendazole was administered orally to five cows. Samples of milk were taken on 1, 3, 5 and 10 days of drug application. The research method includes a description of reagents, plates and equipment; mass-spectrometric conditions for analysis of fenbendazole and its metabolites; preparation of the equipment to operation; preparation of eluent solution; preparation of the chromatograph to analysis; determination of chromatographic parameters of standard drug samples; preparation of milk samples to analysis; establishment of parameters of extracts’ chromatography; procedure of calibration of fenbendazole and its metabolites in eluent.
Results and discussion: When studying the pharmacokinetics of fenbendazole and its metabolites (sulfone and sulfoxide) in milk, it was found that the maximal concentrations were determined 24 h after drug administration and were 22,6 ng/ml for fenbendazole sulfone, 34,0 ng/ml for fenbendazole sulfoxide and 19,7 ng/ml for fenbendazole. 10 days after treatment, the concentrations of fenbendazole and its metabolites in milk did not exceed permitted values.