Open AccessDETECTION OF BABESIA AND ANAPLASMATACEAE BACTERIA IN SMALL MAMMALS FROM IRKUTSK AND NOVOSIBIRSK REGIONS
Author(s) -
В. А. Рар,
O. O. Fedulina,
О. В. Сунцова,
Artem Tikunov,
Victor V. Panov,
О. В. Лисак,
И. В. Козлова,
Nina V. Tikunova
Publication year - 2016
Publication title -
bûlletenʹ vostočno-sibirskogo naučnogo centra
Language(s) - English
Resource type - Journals
ISSN - 1811-0649
DOI - 10.12737/21593
Subject(s) - biology , anaplasmataceae , anaplasma phagocytophilum , babesia , anaplasma , ehrlichia , dna , virology , ixodes persulcatus , genetics , tick , antibody , borrelia burgdorferi
Tissue samples from 228 small mammals captured from 2013 to 2015 in four districts of Irkutsk region and in one
district of Novosibirsk region were examined for the presence of Anaplasmataceae bacteria and Babesia parasites by
nested PCR method with subsequent sequencing of positive samples. In Ekhirit-Bulagatskiy District of Irkutsk region,
Babesia microti DNA was found in 10.9 % of small mammals, Anaplasma phagocytophilum DNA – in 7.3 %, and Ehrlichia
muris DNA – in 1.8 %. Infected mammals were not found in the other three examined districts of Irkutsk region.
In Novosibirsk region, B. microti DNA was found in 8.9 % of small rodents, A. phagocytophilum DNA – in 8.9 % of small
mammals, and Candidatus Neoehrlichia mikurensis DNA – in 8.9 % of small mammals. The identified infection agents
corresponded to microorganism species and genetic variants previously found in Ixodes persulcatus ticks but not in
ticks of other species.