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GALLERIA MELLONELLA L. GARBAGE SORTING MECHANIZATION TECHNOLOGY
Author(s) -
V. D. Sokolov,
А. С. Осокина,
В. В. Касаткин
Publication year - 2020
Publication title -
vestnik kazanskogo gosudarstvennogo agrarnogo universiteta
Language(s) - English
Resource type - Journals
ISSN - 2073-0462
DOI - 10.12737/2073-0462-2020-120-124
Subject(s) - galleria mellonella , larva , biology , sorting , toxicology , environmental science , ecology , computer science , biochemistry , virulence , gene , programming language
In the modern world, insects are widely used for scientific and industrial purposes. For their cultivation in the laboratory, various plants have been developed, the main disadvantage of which is poor-quality sorting from garbage. Therefore, the question of cultivating and sorting the larvae of bee moth (Galleria mellonella L.) in laboratory conditions for the purpose of further use as a model object in various fields of biological sciences and medical purposes is relevant. The effect of the temperature gradient and exposure on the movement of G. mellonella larvae from the heated compartment of the plant was determined by their reproduction into the cold by the number of individuals that moved for 10, 15, and 20 minutes at 35, 40, 45, 50, 55 0С, visual counting. At 35 0С, regardless of the exposure time, the larvae remained on a honeycomb frame; at 40 ° C and 45 0С, on average, 11.5% and 31.8% of individuals, respectively, moved. The higher the temperature gradient, the faster the larvae moved into the cold compartment. More larvae passed from the lower frame to it than from the upper one. The difference at a temperature of 45 ° C averaged 2%, 50 0С - 18.7%, 55 0С - 0.4%. The optimum temperature gradient for sorting larvae is 50 ... 55 0С during an exposure of 15 ... 20 minutes, in this case more than 98% of the larvae were transferred to the cold compartment. The use of an infrared electric heating system will optimize the breeding process of G. mellonella larvae and ensure their high-quality sorting

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