Open AccessFORMATION OF A SYMBIOTIC APPARATUS OF ALFALFA DEPENDING ON THE NITROGEN NUTRITION TYPE
Author(s) -
M.Yu. Kozyreva,
Larisa Basieva
Publication year - 2020
Publication title -
vestnik kazanskogo gosudarstvennogo agrarnogo universiteta
Language(s) - English
Resource type - Journals
ISSN - 2073-0462
DOI - 10.12737/2073-0462-2020-10-16
Subject(s) - rhizobia , inoculation , biology , nitrogen , strain (injury) , symbiosis , chernozem , sowing , agronomy , nitrogen fixation , bacteria , horticulture , botany , soil water , chemistry , ecology , genetics , organic chemistry , anatomy
The experiments were carried out in 2017–2019 in the foothill zone of North Ossetia-Alania (540 m above sea level) on leached chernozem. The effect of the mineral and symbiotrophic types of nitrogen nutrition on the formation of the alfalfa symbiotic apparatus was compared to optimize the cultivation agricultural technique. The experimental design included the following options: control - natural soil fertility; In-1800 - seed treatment with the inoculum of nitrogen-fixing bacteria strains selected in high mountain conditions (1800 m above sea level); Strain 425a - seed inoculation with an industrial strain of rhizotorphin (strain 425a); N30 - starting dose of nitrogen; N30+In - combined use of high-mountain strains of bacteria and starting doses of nitrogen. Seeds were treated with the studied strains before sowing. The formation of nodules on the roots of alfalfa during inoculation of seeds with rhizobia strains occurred 1 ... 2 days earlier than in the control, which indicates their greater activity compared to the local microflora. The duration of the general symbiosis in the second year of life in the variants with inoculation with industrial and alpine strains reached 215 ... 216 days, which is 4 ... 7 days more than in the control and variant N30. The greatest number of nodules in all years was noted during seed treatment with the inoculum of alpine rhizobia strains, both in pure form (96 ... 248 units/plant.), and against the background of the use of starting doses of nitrogen (80 ... 243 units/plant.). The smallest mass of nodules in the experiment (40 ... 255 kg/ha) was recorded in the control and variant N30. The highest mass of nodules was formed by the second crop of the second year of sowing life and exceeded 300 kg/ha in all variants with pre-sowing seed inoculation with active rhizobia strains. The superiority of the In-1800 variant over control was 28.3%, and the differences with N30+In turned out to be insignificant.
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