Open AccessZYMOGRAPHIC ANALYSIS OF LIPOLYTICAL ENZYMES OF SUBCELLULAR VIBRIO CHOLERAE FRACTIONS OF O1 AND O139 SEROGROUPS
Author(s) -
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Е Ю Марков,
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В. Б. Николаев,
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С Н Козлов,
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L. Ya. Urbanovich,
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С К Миткеева
Publication year - 2016
Publication title -
bûlletenʹ vostočno-sibirskogo naučnogo centra
Language(s) - English
Resource type - Journals
ISSN - 1811-0649
DOI - 10.12737/20616
Subject(s) - enzyme , chemistry , agarose , urea , vibrio cholerae , subcellular localization , hydrolysis , biochemistry , membrane , chromatography , specific activity , microbiology and biotechnology , bacteria , biology , gene , genetics
Zymographic analysis showed that all subcellular fractions of the serogroups possessed different degree of lipolytical activity. Maximal hydrolysis in the radial enzyme-diffusion reaction in agarose gel was observed in Tween-20, Tween-80, Triton -305 and Triton - 405. Total hydrolase activity of urea extracts in Tween-20, Tween-80, Triton - 405, Span-85 excelled those in the preparations of outer membranes. Maximal lipolytical activity in regard to the Tweens was shown by the preparations of urea extracts prepared from the nontoxigenic strains of O1 serogroups, while in relation to the Tritons preparations of urea extracts from the toxigenic strains (p < 0,05).