Open AccessExpression of cytochrome P450 aromatase isoforms in female Alburnus tarichi (Guldenstaedtii, 1814)
Author(s) -
Güler Ünal,
Emily Marquez,
Mara H O’Brien,
Pericles Stavropoulos,
İan P. Callard
Publication year - 2021
Publication title -
su ürünleri dergisi
Language(s) - English
Resource type - Journals
eISSN - 2148-3140
pISSN - 1300-1590
DOI - 10.12714/egejfas.38.2.15
Subject(s) - ovary , aromatase , biology , medicine , endocrinology , hypothalamus , endocrine system , population , gene isoform , fish <actinopterygii> , cholesterol side chain cleavage enzyme , clone (java method) , gene , cytochrome p450 , hormone , fishery , genetics , metabolism , environmental health , cancer , breast cancer
In this study, we aimed to clone brain-derived Cyp19b and ovary-derived Cyp19a the P450 aromatase gene isoforms and to indicate the expression levels of these genes in the hypothalamus and ovary tissues from reproductively arrested ovarian development (RA) and non-arrested ovarian development (RN) Alburnus tarichi from Lake Van, Turkey. The expression levels of Cyp19b and Cyp19a genes were predominant in the hypothalamus and ovary, respectively. The level of Cyp19b mRNA was significantly lower in the hypothalamus and ovary from RA fish than in the hypothalamus and ovary from RN fish (P<0.05). The expression level of Cyp19a was significantly lower in the ovary of RA fish (P<0.05) than RN fish while no difference was found in the hypothalamus of both RA and RN fish (P<0.05). According to these findings, we suggest that the RA fish represent a segment of the population and these fish may be more sensitive to endocrine disruption compound/s than others.