Purification and Characterization of β-Glucosidase Involved in the Emission of 2-Phenylethanol from Rose Flowers | Zendy

Miwa Sakai | Zendy; Saori Tomita | Zendy; Hiroshi Hirata | Zendy; Tatsuo Asai | Zendy; Hideo Dohra | Zendy; Masakazu Hara | Zendy; Naoharu Watanabe | Zendy

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Purification and Characterization of β-Glucosidase Involved in the Emission of 2-Phenylethanol from Rose Flowers

Author(s) -

Miwa Sakai,

Saori Tomita,

Hiroshi Hirata,

Tatsuo Asai,

Hideo Dohra,

Masakazu Hara,

Naoharu Watanabe

Publication year - 2008

Publication title -

bioscience biotechnology and biochemistry

Language(s) - English

Resource type - Journals

SCImago Journal Rank - 0.509

H-Index - 116

eISSN - 1347-6947

pISSN - 0916-8451

DOI - 10.1271/bbb.70404

Subject(s) - petal , rose (mathematics) , enzyme , chemistry , beta glucosidase , botany , biochemistry , stereochemistry , biology , enzyme assay , horticulture

Beta-glucosidase was partially purified from Rosa 'Hoh-Jun' petals. The enzyme was highly specific for such beta-D-glucopyranosides as 2-phenylethyl beta-D-glucopyranoside. The optimal activity was observed at pH 6.0 and 35 degrees C. The enzymes were composed with two proteins (160 and 155 kDa) by blue native-PAGE, and were classified in a family 1 glucosidase based on LC-MS/MS analyses.

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