Open AccessInduction of oestrus in ewes of the rare Greek breeds Skopelos, Zakynthos, Kymi - Electrical resistance of cervical mucous
Author(s) -
Theodora Tsiligianni
Publication year - 2017
Publication title -
journal of the hellenic veterinary medical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.186
H-Index - 7
eISSN - 2585-3724
pISSN - 1792-2720
DOI - 10.12681/jhvms.15509
Subject(s) - estrous cycle , medroxyprogesterone acetate , endocrinology , medicine , zoology , biology , estrogen
Objective of the study was the investigation of the response of Skopelos, Zakynthos and Kymi ewes to oestrus induction treatment. Furthermore, feasibility of measuring electrical resistance of cervical mucus for oestrus detection was examined. Oestrus was induced by intravaginal insertion of sponges impregnated with 60 mg medroxyprogesterone acetate for 12 days [groups: Skopelos (n=17), Zakynthos (n=18), Kymi (n=22)], followed by intramuscular injection of 400 iu equine chorionic gonadotropins) at the time of withdrawal. Blood samples were collected 10 days before sponge insertion, at insertion and withdrawal and at oestrus for measurement of oestradiol 17-β and progesterone concentration. Electrical resistance of cervical mucus was measured after οestrus detection, then ewes were mated. Proportion of Zakynthos ewes (100%) in oestrus was greater than that of Kymi (64%) or Skopelos (78%) ewes. Pregnancy rate ranged from 50% (Kymi ewes) to 67% (Skopelos ewes). Electrical resistance of cervical mucus of ewes that conceived was significantly smaller than of those that did not. Based on results of progesterone blood concentration measurement 10 days before and at sponge insertion, only Kymi ewes were at full anoestrus, whilst Zakynthos and Skopelos ewes were in the breeding season. Progesterone blood concentration at sponge withdrawal and oestradiol 17-β blood concentration at oestrus were significantly greater in Zakynthos ewes that conceived compared to those in ewes that did not conceive. Furthermore, progesterone concentration at sponge insertion was significantly greater in all ewes that conceived compared to that in ewes that did not. There were significant differences in oestradiol-17β and progesterone blood concentrations among breeds and at the various time-points of blood collection. It is concluded that response of the above sheep breeds to oestrus induction was satisfactory. Hormonal status of ewes during sponge insertion is of importance for conception. Measurement of electrical resistance of cervical mucus could be useful for selection of ewes for mating, even if rams used to detect oestrous.