Open AccessFreezing of rabbit semen after the addition of glycerol and dimethylsulfoxide in the extender
Author(s) -
S. Samouilidis,
K. Saoulidis,
A. Foukos,
Petros Ypsilantis,
A. Demertzis,
A. P. Sbiraki
Publication year - 2018
Publication title -
journal of the hellenic veterinary medical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.186
H-Index - 7
eISSN - 2585-3724
pISSN - 1792-2720
DOI - 10.12681/jhvms.15461
Subject(s) - extender , semen , sperm motility , sperm , zoology , andrology , semen extender , artificial insemination , glycerol , chemistry , insemination , yolk , biology , food science , biochemistry , medicine , pregnancy , genetics , organic chemistry , polyurethane
In this study, semen from 10 rabbits was frozen after it was diluted in two different extenders, A and B. Extender A consisted of Tris-buffer in which 20% of egg yolk and 3% of glycerol were added, while extender Β had the same composition, plus 3% of dimethylsulfoxide (DMSO). The semen of the buck exhibiting the best post-thaw motility in the two extenders, was used for the insemination of two corresponding groups (I and II) of 20 does each. The average sperm motility of fresh semen (77%) was significantly reduced (P 0.05). Also, no significant difference (P>0.05) was observed between the average litter size of group I (6,8±1,7) and group II (7,0±1,9). Thereafter, it is concluded that freezing rabbit semen in an extender that contains a combination of the cryoprotective agents glycerol and DMSO, in the proportion of 3% and 3%, respectively, results in a significant improvement of post-thaw sperm motility, while it doesn't affect significantly its fertility value neither the size of the litters.