Open AccessDifferentiation of leptospiral serovars by restriction endonucleases analysis
Author(s) -
Ι. Μ. Eljalii,
Abdelwahid Saeed Ali
Publication year - 2017
Publication title -
journal of the hellenic veterinary medical society
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.186
H-Index - 7
eISSN - 2585-3724
pISSN - 1792-2720
DOI - 10.12681/jhvms.15060
Subject(s) - restriction enzyme , serotype , biology , strain (injury) , microbiology and biotechnology , restriction map , dna , restriction site , restriction fragment length polymorphism , genetics , genotype , gene , base sequence , anatomy
DNA from ten leptospiral field isolates was digested with restriction enzymes, Hindlll, BamHl and EcoRl to differentiate leptospiral serovars. A high heterogeneity among the serovars examined was revealed. Similarities were observed among isolates of the same serovar. No relationship was found between the restriction patterns and the species from which the field strain was isolated. It appears that restriction endonucleases analysis would be useful in the differentiation of the leptospiral serovars.