Open AccessDistribution and Community Composition of Anammox Bacteria in Shallow Groundwater of the Kathmandu Valley, Nepal
Author(s) -
Mai Nakano,
Takeshi Kamei,
Bijay Man Shakya,
Takashi Nakamura,
Yasuhiro Tanaka,
Eiji Haramoto,
Tadashi Toyama,
Futaba Kazama
Publication year - 2021
Publication title -
microbes and environments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.048
H-Index - 50
eISSN - 1347-4405
pISSN - 1342-6311
DOI - 10.1264/jsme2.me20143
Subject(s) - anammox , groundwater , distribution (mathematics) , geography , composition (language) , environmental science , ecology , biology , geology , chemistry , nitrogen , mathematics , geotechnical engineering , denitrifying bacteria , organic chemistry , denitrification , mathematical analysis , linguistics , philosophy
The abundance and diversity of anaerobic ammonium oxidation (anammox) bacteria were assessed in 152 groundwater samples in the Kathmandu Valley, Nepal. Anammox bacterial 16S rRNA genes were detected in 54% (37/68) of samples collected in the dry season at 1.6×10 5 -8.8×10 6 copies L -1 , and in 60% (50/84) of samples collected in the wet season at 4.3×10 4 -1.2×10 7 copies L -1 . The 16S rRNA genes of "Candidatus Brocadia", "Candidatus Anammoxoglobus", and five new deduced anammox bacterial phylotypes were detected in the shallow groundwater samples. Diverse anammox bacteria were broadly distributed in the shallow groundwater aquifer of the Kathmandu Valley.