Open AccessMicrobial Community Analysis of Anaerobic Enrichment Cultures Supplemented with Bacterial Peptidoglycan as the Sole Substrate
Author(s) -
Samia Quaiyum,
Kensuke Igarashi,
Takashi Narihiro,
Souichiro Kato
Publication year - 2020
Publication title -
microbes and environments
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.048
H-Index - 50
eISSN - 1347-4405
pISSN - 1342-6311
DOI - 10.1264/jsme2.me20002
Subject(s) - peptidoglycan , micrococcus luteus , biology , verrucomicrobia , microbiology and biotechnology , anaerobic exercise , bacteroidetes , bacteria , escherichia coli , food science , biochemistry , 16s ribosomal rna , physiology , genetics , gene
Methanogenic microbial communities were enriched from rice paddy soil and anaerobic digester sludge using peptidoglycan purified from gram-negative Escherichia coli or gram-positive Micrococcus luteus as the sole substrate. Methane production data suggested the anaerobic degradation of peptidoglycan and also that peptidoglycan from E. coli had lower degradability. The community structures of enrichment cultures fed peptidoglycan from E. coli or M. luteus were similar, but distinctly different. A number of phylogenetically novel and uncultured bacteria, particularly in the phyla Bacteroidetes, WWE1, Armatimonadetes, and Verrucomicrobia, dominated the enrichment cultures, suggesting their involvement in anaerobic peptidoglycan degradation.