Open AccessAssessment of Na+/K+ ATPase Activity in Small Rodent and Human Skeletal Muscle Samples
Author(s) -
Sebastián Jannas-Vela,
Stuart Brownell,
Heather L. Petrick,
George J. F. Heigenhauser,
Lawrence L. Spriet,
Graham P. Holloway
Publication year - 2019
Publication title -
medicine and science in sports and exercise
Language(s) - English
Resource type - Journals
eISSN - 1530-0315
pISSN - 0195-9131
DOI - 10.1249/mss.0000000000002063
Subject(s) - serca , skeletal muscle , myosin , atpase , myosin atpase , ouabain , chemistry , muscle contraction , medicine , biochemistry , biology , endocrinology , enzyme , sodium , organic chemistry
In skeletal muscle, the Na/K ATPase (NKA) plays essential roles in processes linked to muscle contraction, fatigue, and energy metabolism; however, very little information exists regarding the regulation of NKA activity. The scarcity of information regarding NKA function in skeletal muscle likely stems from methodological constraints, as NKA contributes minimally to total cellular ATP utilization, and therefore contamination from other ATPases prevents the assessment of NKA activity in muscle homogenates. Here we introduce a method that improves accuracy and feasibility for the determination of NKA activity in small rodent muscle samples (5-10 mg) and in human skeletal muscle.