Open AccessStrain specific differences in muscle Ca2+ transport and mitochondrial electron transport proteins between FVB/N and C57BL/6J mice
Author(s) -
Sushant Singh,
Muthu Periasamy,
Naresh C. Bal
Publication year - 2020
Publication title -
journal of experimental biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.367
H-Index - 185
eISSN - 1477-9145
pISSN - 0022-0949
DOI - 10.1242/jeb.238634
Subject(s) - mitochondrion , strain (injury) , skeletal muscle , inbred strain , biology , electron transport chain , gene expression , metabolism , phenotype , gene , microbiology and biotechnology , biochemistry , anatomy
Genetically engineered mouse models have been used to determine the role of sarcolipin (SLN) in muscle. However, few studies had difficulty in detecting SLN in FBV/N mice and questioned its relevance to muscle metabolism. It is known that genetic alteration of proteins in different inbred mice strains produce dissimilar functional outcome. Therefore, here we compared the expression of SLN and key proteins involved in Ca2+-handling and mitochondrial metabolism between FVB/N and C57BL/6J mouse strains. Data suggests that, SLN expression is less abundant in the skeletal muscles of FVB/N mice compared to C57BL/6J strain. The expression of Ca2+-transporters in the mitochondrial membranes, were also lower in FVB/N compared to C57BL/6J. Similarly the electron transport proteins in the mitochondria were less abundant in FVB/N mice which may contribute to differences in energy metabolism. Future studies using different mouse strains should take these differences into account while interpreting their data.