Thermal stress causes DNA damage and mortality in a tropical insect

Cold tolerance is considered an important factor determining geographic distribution of insects. We've previously shown that despite tropical origin, cockroach Gromphadorinha coquereliana is capable of surviving exposures to cold. However, freezing tolerance of this species had not yet been examined. Low temperature is known to alter membranes integrity in insects but whether chilling or freezing compromises DNA integrity remains a matter of speculation. In the present study, we subjected the G. coquereliana adults to freezing to determine their supercooling point (SCP) and evaluated whether the cockroaches were capable of surviving partial and complete freezing. Next, we conducted single cell gel electrophoresis assay (SCGE) to determine whether heat, cold and freezing altered haemocytes DNA integrity. The SCP of this species was high and around -4.76°C, which is within typical range of freezing-tolerant species. Most cockroaches survived one day after partial ice formation (20% mortality), but died progressively in the next few days after cold stress (70% mortality after 4 days). One day after complete freezing, most insects died (70% mortality), and after 4 days, 90% of them had succumbed. The SCGE assays showed substantial level of DNA damage in haemocytes. When cockroaches were heat-stressed, the level of DNA damage was similar to that observed in the freezing treatment; though all heat-stressed insects survived. The study shows that G. coquereliana can surprisingly be considered as moderately freezing-tolerant species, and for first time that extreme low temperature stress can affect DNA integrity, suggesting that this cockroach may possess an efficient DNA repair system.