Open AccessChanges in free amino acid concentrations and associated gene expression profiles in the abdominal muscle of kuruma shrimp Marsupenaeus japonicus acclimated at different salinities
Author(s) -
Hiroki Koyama,
Naoki Mizusawa,
Masataka Hoashi,
Engkong Tan,
Ko Yasumoto,
Mitsuru Jimbo,
Daisuke Ikeda,
Takashi Yokoyama,
Shuichi Asakawa,
Sanit Piyapattanakorn,
Shugo Watabe
Publication year - 2018
Publication title -
journal of experimental biology
Language(s) - English
Resource type - Journals
eISSN - 1477-9145
pISSN - 0022-0949
DOI - 10.1242/jeb.168997
Subject(s) - amino acid , biology , glutamine , salinity , acclimatization , biochemistry , shrimp , osmoregulation , metabolism , alanine , gene expression , gene , ecology
Shrimps inhabiting the coastal water can survive in a wide range of salinity. However, the molecular mechanisms involved in their acclimation to different environmental salinities have remained largely unknown. In the present study, we acclimated kuruma shrimp Marsupenaeus japonicus at 1.7 %, 3.4 % and 4.0 % salinities. After acclimating for 6, 12, 24 and 72 h, we determined free amino acid concentrations in their abdominal muscle, and performed RNA-seq analysis on this muscle. The concentrations of free amino acids were clearly altered depending on salinity after acclimating for 24 h. Glutamine and alanine concentrations were markedly increased following the increase of salinity. In association with such changes, many genes related to amino acid metabolism changed their expression levels. In particular, the increase of the expression level of the gene encoding glutamate-ammonia ligase which functions in the glutamine metabolism appeared to be relevant to the increased glutamine concentration at high salinity. Furthermore, the alanine concentration increased at high salinity was likely to be associated with the decrease in the expression levels of the alanine-glyoxylate transaminase gene. Thus, there is a possibility that changes in the concentration of free amino acids for osmoregulation in kuruma shrimp are regulated by changes in the expression levels of genes related to amino acid metabolism.