
miR-210 expression is associated with methionine-induced differentiation of trout satellite cells
Author(s) -
Mary N. Latimer,
Nathalie Sabin,
Aurélie Le Cam,
Iban Seiliez,
Peggy R. Biga,
Jean-Charles Gabillard
Publication year - 2017
Publication title -
journal of experimental biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.367
H-Index - 185
eISSN - 1477-9145
pISSN - 0022-0949
DOI - 10.1242/jeb.154484
Subject(s) - myogenin , myogenesis , microrna , methionine , meth , biology , downregulation and upregulation , cellular differentiation , microbiology and biotechnology , chemistry , genetics , myocyte , gene , amino acid , monomer , organic chemistry , acrylate , polymer
In fish, data on miRNAs involved in myogenesis are scarce. In order to identify miRNAs involved in satellite cell differentiation, we used a methionine depletion/replenishment protocol to synchronize myogenic cell differentiation. Our results validated that methionine removal (72H) from the medium strongly decreased myoD1 and myogenin expression indicating differentiation arrest. In contrast, methionine replenishment rescued expression of myoD1 and myogenin showing a resumption of differentiation. We performed a miRNA array analysis of myogenic cells from three conditions: presence of methionine (CTRL), absence of methionine during 72h (Meth-) and absence of methionine during 48H with 24H of methionine replenishment (Meth -/+). A clustering analysis identified three clusters: cluster I corresponds to miRNA upregulated only in Meth -/+ conditions; cluster II corresponds to miRNA downregulated only in Meth -/+ conditions; cluster III corresponds to miRNAs with high expression in control, low expression in absence of methionine (Meth -) and middle expression after methionine replenishment (Meth -/+). Cluster III was very interesting because it fit with the data obtained for myoD1 and myogenin (supporting an involvement in the differentiation) and contained 7 miRNAs with muscle-related function (e.i. miR-133a) and one (miR-210) with unknown function. Based on our already published miRNAs repertoire (Juanchich et al., 2016), we confirmed miR-133a had expression only in white muscle and showed that miR-210 had strong expression in white muscle. We also showed that miR-210 expression was upregulated during differentiation of satellite cells suggesting that miR-210 was potentially involved in the differentiation of satellite cells.